Coagulase-negative staphylococcal skin carriage among neonatal intensive care unit personnel: from population to infection.
نویسندگان
چکیده
Coagulase-negative staphylococci (CoNS) are a major cause of sepsis in neonatal intensive care units (NICU) worldwide. Infecting strains of these commensal bacteria may originate from NICU personnel. Therefore, we studied the characteristics of CoNS isolates from NICU personnel and compared them to those of isolates from the general population and from sepsis patients. Furthermore, we studied the epidemiological effect on CoNS carriage of NICU personnel after a period of absence. In our study, we isolated CoNS from the thumbs of NICU personnel every 2 weeks during the summer of 2005 and sampled personnel returning from vacation and a control group from the general population. Furthermore, we collected sepsis isolates from this period. Isolates were tested for antibiotic resistance, mecA and icaA carriage, biofilm production, and genetic relatedness. We found that mecA and icaA carriage as well as penicillin, oxacillin, and gentamicin resistance were significantly more prevalent in CoNS strains from NICU personnel than in community isolates. Similar trends were observed when postvacation strains were compared to prevacation strains. Furthermore, genetic analysis showed that 90% of the blood isolates were closely related to strains found on the hands of NICU personnel. Our findings revealed that CoNS carried by NICU personnel differ from those in the general population. Hospital strains are replaced by community CoNS after a period of absence. NICU personnel are a likely cause for the cross-contamination of virulent CoNS that originate from the NICU to patients.
منابع مشابه
Coagulase - negative staphylococcal skin carriage among NICU personnel : from 1 population to infection
متن کامل
Prevalence of molecular types and mecA gene carriage of coagulase-negative Staphylococci in a neonatal intensive care unit: relation to nosocomial septicemia.
Molecular typing of isolates revealed that neonatal coagulase-negative staphylococcal (CONS) septicemia is most frequently caused by predominant, antibiotic-resistant CONS types, which are widely distributed among both neonates and staff of the neonatal unit, suggesting cross-contamination. Therefore, infection control measures may be valuable in the prevention of this common nosocomial septice...
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متن کاملGenetic population structure of coagulase-negative staphylococci associated with carriage and disease in preterm infants.
Coagulase-negative staphylococci (CoNS) are a leading cause of sepsis in the neonatal intensive care unit (NICU) setting. To evaluate the hypothesis that isolates of CoNS associated with disease belong to hypervirulent clones, as opposed to being drawn randomly from the neonatal unit carriage flora, we conducted a prospective, case-controlled study in a busy NICU. Using pulsed-field gel electro...
متن کاملMolecular epidemiology of coagulase - negative Staphylococcus carriage in 1 neonates admitted to an intensive care unit in Brazil 2
18 Background 19 Nasal colonization with coagulase-negative Staphylococcus (CoNS) has been 20 described as a risk factor for subsequent systemic infection. In this study, we aimed to 21 evaluate the profile of CoNS isolates colonizing the nares of children admitted to a 22 neonatal intensive care unit (NICU) of a major city located in central Brazil. 23 Methods 24 We assessed CoNS carriage at a...
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 48 11 شماره
صفحات -
تاریخ انتشار 2010